AUTOMATED RAMAN IMAGING MICROSCOPE (alpha300 apyron) – OXFORD INSTRUMENTS (WITec)

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The alpha300 apyron is an advanced Raman microscope system that fully automates the control and measurement processes, saving time and enhancing accuracy.
With automated measurement capabilities and high-level optimization, the alpha300 apyron delivers Raman images with exceptional sensitivity and resolution, making it an ideal solution for material research and analytical applications.

AUTOMATED RAMAN IMAGING MICROSCOPE (alpha300 apyron) – OXFORD INSTRUMENTS (WITec)

Setup and Configuration
  • TrueCal executes pre-configured calibration procedures.
  • Compliant with laser safety standards.
  • The AutoBeam Concept enables automated and motorized beam path alignment, including:
  1. Motorized 6-position objective turret: selects objective lenses and compensates for deviations.
  2. Motorized illumination selector: switches between all microscope illumination options.
  3. Motorized laser coupler: supports up to 6 laser wavelengths from UV to NIR.
  4. AutoBeam coupler: automatically optimizes signal intensity and resolution, and selects the appropriate spectrometer.
  5. Motorized calibration source: enables fast, automatic spectrometer calibration at multiple points across the entire spectral range.
  6. Motorized Köhler illumination aperture: supports focusing and optimizes contrast and uniformity.
  7. Motorized polarization modules: excitation polarizer and detection analyzer with fully automated rotation.
Raman Imaging
  • TruePower sets and determines absolute laser power with an accuracy of <0.1 mW.
  • The system automatically repositions samples via motorized scanning stages and piezo drives.
  • Focus Stabilization actively finds and maintains focus throughout the measurement.
  • The motorized objective turret includes precise compensation features and is compatible with AFM/SNOM objectives.
  • The EasyLink handheld controller enables intuitive device operation.
Data Processing and Visualization
  • Suite SIX software guides users through Raman spectral processing, from background subtraction to image generation.
  • TrueComponent Analysis automatically identifies sample components within an image and distinguishes their individual spectra.
  • TrueMatch software identifies molecules by comparing measured spectra with existing databases.

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The Raman Imaging Microscope – alpha300 R is an advanced instrument that integrates optical microscopy with Raman spectroscopy technology, offering exceptional performance in sensitivity, resolution, and speed. This system enables hyperspectral imaging with complete Raman spectral information at every pixel, making it ideal for 3D imaging and depth profiling. Equipped with an optimized optical system and UHTS (Ultra-High Throughput Spectrometer) designed for low-light intensity applications, this microscope provides a non-destructive and highly precise solution for demanding research without compromising the integrity of the sample.